Format

Send to

Choose Destination
See comment in PubMed Commons below
Bioconjug Chem. 2013 Sep 18;24(9):1445-54. doi: 10.1021/bc400291f. Epub 2013 Sep 3.

Rational design of chemical ligands for selective mitochondrial targeting.

Author information

1
Department of Biochemistry and Microbiology and§Department of Analytical Chemistry, Institute of Chemical Technology in Prague , Technická 5, 166 28, Prague 6, Czech Republic.

Abstract

The rational design of molecules with selective intracellular targeting is a great challenge for contemporary chemistry and life sciences. Here, we demonstrate a rational approach to development of compartment-specific fluorescent dyes from the γ-aryl substituted pentamethine family. These novel dyes exhibit an extraordinary affinity and selectivity for cardiolipin in inner mitochondrial membrane and possess excellent photostability, fluorescent properties, and low phototoxicity. Selective imaging of live and fixed mitochondria was achieved in various cell lines using nanomolar concentrations of these dyes. Their high localization specificity and low toxicity enables study of morphological changes, structural complexity, and dynamics of mitochondria playing a pivotal role in many pathological diseases. These far-red emitting dyes could also serve in a variety of biomedical applications.

PMID:
23961900
DOI:
10.1021/bc400291f
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for American Chemical Society
    Loading ...
    Support Center