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Angew Chem Int Ed Engl. 2013 Sep 27;52(40):10593-7. doi: 10.1002/anie.201304096. Epub 2013 Aug 19.

Bioorthogonal approach to identify unsuspected drug targets in live cells.

Author information

1
Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge Street, Boston, MA 02114 (USA).

Abstract

A proteomics method to pull down secondary drug targets from live cells is described. The drug of interest is modified with trans-cyclooctene (TCO) and incubated with live cells. Upon cell lysis, the modified drug bound to the protein is pulled down using magnetic beads decorated with a cleavable tetrazine-modified linker. Samples are then run on an SDS-PAGE gel and isolated bands are submitted for mass spectrometry analysis to identify drug targets.

KEYWORDS:

bioorthogonal reaction; cleavable linkers; drug targets; proteins; proteomics

PMID:
23960025
PMCID:
PMC3856564
DOI:
10.1002/anie.201304096
[Indexed for MEDLINE]
Free PMC Article
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