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Methods Mol Biol. 2013;1035:121-33. doi: 10.1007/978-1-62703-508-8_10.

The prospective isolation of viable, high ploidy megakaryocytes from adult murine bone marrow by fluorescence activated cell sorting.

Author information

1
Materials Science and Engineering, Commonwealth Scientific and Industrial Research Organization, Melbourne, VIC, Australia.

Abstract

Mature megakaryocytes (MM) can be up to 65 μM in diameter and due to their size, viable and pure MM populations have been difficult to isolate in large numbers. Here in, we report a fluorescence activated cell sorting (FACS) method by which viable and pure populations of 8 N, 16 N, 32 N, and 64 N MM can be isolated from murine bone marrow (BM).

PMID:
23959986
DOI:
10.1007/978-1-62703-508-8_10
[Indexed for MEDLINE]

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