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Insect Sci. 2014 Feb;21(1):20-30. doi: 10.1111/1744-7917.12026. Epub 2013 Jun 22.

Expression analysis of chlorophyllid α binding protein, a secretory, red fluorescence protein in the midgut of silkworm, Bombyx mori.

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1
Institute of Sericulture and Apiculture, College of Animal Sciences, Zhejiang University, Hangzhou, China.

Abstract

Chlorophyllid α binding protein (chbp) was recently characterized by its ability to bind the prosthetic group of chlorophylls and little information is known regarding its expression. In the present study, we found that chpb was expressed highly and exclusively in the midgut of silkworm, Bombyx mori. The expression level of chbp was very high in the newly molted fifth instar larvae followed by gradual decline in the same instar. Our results demonstrated that CHBP was a secretory protein and located mainly in the apical of midgut epithelial cells. Real-time polymerase chain reaction analysis results showed that chpb highly expressed in the anterior midgut, threefold and sixfold higher compared with that of the middle midgut and posterior midgut, respectively, and chpb expression declined in darkness. In addition, the expression of chbp was affected by high-dose virus or bacterium infection.

KEYWORDS:

Bombyx mori; chlorophyllid α binding protein; expression; midgut

PMID:
23956194
DOI:
10.1111/1744-7917.12026
[Indexed for MEDLINE]
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