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Nat Methods. 2013 Oct;10(10):992-5. doi: 10.1038/nmeth.2605. Epub 2013 Aug 18.

Recombinant antibodies to histone post-translational modifications.

Author information

1
Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois, USA.

Abstract

Variability in the quality of antibodies to histone post-translational modifications (PTMs) is a widely recognized hindrance in epigenetics research. Here, we produced recombinant antibodies to the trimethylated lysine residues of histone H3 with high specificity and affinity and no lot-to-lot variation. These recombinant antibodies performed well in common epigenetics applications, and enabled us to identify positive and negative correlations among histone PTMs.

PMID:
23955773
PMCID:
PMC3828030
DOI:
10.1038/nmeth.2605
[Indexed for MEDLINE]
Free PMC Article

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