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Nat Methods. 2013 Oct;10(10):996-8. doi: 10.1038/nmeth.2604. Epub 2013 Aug 18.

UPARSE: highly accurate OTU sequences from microbial amplicon reads.

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1
Independent Investigator, Tiburon, California, USA.

Abstract

Amplified marker-gene sequences can be used to understand microbial community structure, but they suffer from a high level of sequencing and amplification artifacts. The UPARSE pipeline reports operational taxonomic unit (OTU) sequences with ≤1% incorrect bases in artificial microbial community tests, compared with >3% incorrect bases commonly reported by other methods. The improved accuracy results in far fewer OTUs, consistently closer to the expected number of species in a community.

PMID:
23955772
DOI:
10.1038/nmeth.2604
[Indexed for MEDLINE]
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