Format

Send to

Choose Destination
Nat Methods. 2013 Oct;10(10):981-4. doi: 10.1038/nmeth.2603. Epub 2013 Aug 18.

Site-specific characterization of the Asp- and Glu-ADP-ribosylated proteome.

Author information

1
Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas, USA.

Abstract

Poly(ADP-ribosyl)ation is catalyzed by a family of enzymes known as PARPs. We describe a method to characterize the human aspartic acid- and glutamic acid-ADP-ribosylated proteome. We identified 1,048 ADP-ribosylation sites on 340 proteins involved in a wide array of nuclear functions; among these were many previously unknown PARP downstream targets whose ADP-ribosylation was sensitive to PARP inhibitor treatment. We also confirmed that iniparib had a negligible effect on PARP activity in intact cells.

PMID:
23955771
DOI:
10.1038/nmeth.2603
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center