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J Ethnopharmacol. 2013 Oct 28;150(1):389-92. doi: 10.1016/j.jep.2013.08.009. Epub 2013 Aug 14.

Modulation effect of Smilax glabra flavonoids on ryanodine receptor mediated intracellular Ca2+ release in cardiomyoblast cells.

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Experimental Animal Research Center, Zhejiang Chinese Medical University, Hangzhou 310053, China.



Smilax glabra rhizome, a plant material from Liliaceae family, is a widely used traditional Chinese medicine for anti-cardiac hypertrophy treatment. We have previously found that Smilax glabra flavonoids (SGF) exerted such anti-cardiac hypertrophy activity. However, the mechanism of this activity of SGF has not been clarified yet.


This study was aimed to investigate the inhibitory role of SGF on intracellular Ca(2+) release in rat cardiomyoblast cells (H9C2). Intracellular Ca(2+) release was determined by Ca(2+) indicator fluorescence (fluo 4-AM) in H9C2 cell line.


SGF at concentrations of 0.25, 0.5, 1.0mg/ml significantly inhibited the phenylephrine or angiotensin II induced intracellular Ca(2+) release in a dose-dependent manner. Furthermore, SGF could also inhibit ryanodine receptor (RyR) agonist caffeine induced Ca(2+) release and phenylephrine (PE)-induced Ca(2+) release under the condition in which inositol trisphosphate (IP3) receptors were blocked with 2-Aminoethoxydiphenyl borate (2-APB). Nevertheless, SGF had no impact on PE-induced Ca(2+) release under the condition in which RyRs were blocked with tetracaine.


Our results suggest that the protective effects of SGF are mediated via targeting inhibition of RyR mediated intracellular Ca(2+) release.


2-APB; 2-Aminoethoxydiphenyl borate; ANP; Ang II; Angiotensin II; CICR; Ca(2+)-induced Ca(2+) release; ER; FCS; HBSS; Hanks balanced salt solution; IP3; IP3R; Inositol 1,4,5-trisphosphate receptors; Intracellular Ca(2+) release; PE; RyR; RyR2; Ryanodine receptors; SGF; SR; Smilax glabra flavonoids; atrial natriuretic peptide; endoplasmic reticulum; fetal calf serum; flavonoids of Smilax glabra; inositol trisphosphate; inositol trisphosphate receptor; phenylephrine; ryanodine receptor; ryanodine receptor 2; sarcoplasmic reticulum

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