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Angew Chem Int Ed Engl. 2013 Sep 27;52(40):10487-90. doi: 10.1002/anie.201304391. Epub 2013 Aug 14.

Efficient detection of hydrogen bonds in dynamic regions of RNA by sensitivity-optimized NMR pulse sequences.

Author information

1
Institute of Structural Biology, Helmholtz Zentrum München, Ingolstädter Landstr. 1, 85764 Neuherberg (Germany) http://www.nmr.ch.tum.de/; Center for Integrated Protein Science Munich and Chair of Biomolecular NMR, TU München, Lichtenbergstr. 4, 85747 Garching (Germany).

Abstract

Improved Sensitivity: Efficient NMR experiments are presented for determining the secondary structure in large and dynamic RNAs using J-couplings across hydrogen bonds. The experiments provide up to eight-fold improved sensitivity and thus enable detection of base pairs in dynamic regions even in large RNAs.

KEYWORDS:

NMR spectroscopy; RNA dynamics; RNA secondary structure; base pairs; hydrogen bonds

PMID:
23946052
PMCID:
PMC3882076
DOI:
10.1002/anie.201304391
[Indexed for MEDLINE]
Free PMC Article

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