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Angew Chem Int Ed Engl. 2013 Sep 27;52(40):10553-8. doi: 10.1002/anie.201304038. Epub 2013 Aug 13.

DNA modification under mild conditions by Suzuki-Miyaura cross-coupling for the generation of functional probes.

Author information

1
Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford OX1 3TA (UK).

Abstract

Quick and clean: A method for Pd-catalyzed Suzuki-Miyaura cross-coupling to iododeoxyuridine (IdU) in DNA is described. Key to the reactivity is the choice of the ligand and the buffer. A covalent [Pd]-DNA intermediate was isolated and characterized. Photocrosslinking probes were generated to trap proteins that bind to epigenetic DNA modifications.

KEYWORDS:

DNA; cross-coupling; oligonucleotides; palladium; photoaffinity labeling

PMID:
23943570
PMCID:
PMC3823066
DOI:
10.1002/anie.201304038
[Indexed for MEDLINE]
Free PMC Article

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