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Muscle Nerve. 2013 Nov;48(5):745-51. doi: 10.1002/mus.23805. Epub 2013 Aug 13.

Reduced DHPRα1S and RyR1 expression levels are associated with diaphragm contractile dysfunction during sepsis.

Author information

1
Respiratory Department and Intensive Care Unit, Shengjing Hospital of China Medical University, Shenyang, Postal Code, 110004, People's Republic of China.

Abstract

INTRODUCTION:

Sepsis often causes diaphragm contractile dysfunction. Dihydropyridine receptors (DHPRα1s and DHPRα1c) and ryanodine receptors (RyR1, RyR2, and RyR3) are essential for excitation-contraction coupling in striated muscles. However, their expression in diaphragm during sepsis have not been explored.

METHODS:

Eight rats received endotoxin, and 8 more rats received placebo. After 24 hours, 3) diaphragm isometric contractile force was measured. The mRNA and protein levels of DHPRs and RyRs in diaphragm muscles were determined.

RESULTS:

Sepsis weakened diaphragm contractile function. The expression levels of DHPRα1s and RyR1 were significantly lower in septic rats than in control rats. The expression levels of DHPRα1c and RyR3 were unaffected by sepsis. RyR2 was undetectable at both mRNA and protein levels in the control and sepsis groups.

CONCLUSIONS:

Weakened diaphragm contraction in the septic rats was associated with reduced mRNA and protein expression of DHPRα1s and RyR1, the isoforms of skeletal muscles.

KEYWORDS:

DHPR; RyR; diaphragm muscle; excitation-contraction coupling; sepsis

PMID:
23943510
DOI:
10.1002/mus.23805
[Indexed for MEDLINE]
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