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Bioresour Technol. 2013 Oct;146:267-273. doi: 10.1016/j.biortech.2013.07.078. Epub 2013 Jul 25.

Simultaneous saccharification and fermentation of Agave tequilana fructans by Kluyveromyces marxianus yeasts for bioethanol and tequila production.

Author information

1
Unidad de Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Avenida Normalistas 800, Col. Colinas de la Normal, C.P. 44270 Guadalajara, Jal., Mexico.
2
Unidad de Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Avenida Normalistas 800, Col. Colinas de la Normal, C.P. 44270 Guadalajara, Jal., Mexico. Electronic address: jparrizon@ciatej.net.mx.

Abstract

Agave tequilana fructans (ATF) constitute a substrate for bioethanol and tequila industries. As Kluyveromyces marxianus produces specific fructanases for ATF hydrolysis, as well as ethanol, it can perform simultaneous saccharification and fermentation. In this work, fifteen K. marxianus yeasts were evaluated to develop inoculums with fructanase activity on ATF. These inoculums were added to an ATF medium for simultaneous saccharification and fermentation. All the yeasts, showed exo-fructanhydrolase activity with different substrate specificities. The yeast with highest fructanase activity in the inoculums showed the lowest ethanol production level (20 g/l). Five K. marxianus strains were the most suitable for the simultaneous saccharification and fermentation of ATF. The volatile compounds composition was evaluated at the end of fermentation, and a high diversity was observed between yeasts, nevertheless all of them produced high levels of isobutyl alcohol. The simultaneous saccharification and fermentation of ATF with K. marxianus strains has potential for industrial application.

KEYWORDS:

Agave tequilana fructans; Bioethanol; Kluyveromyces marxianus; Saccharification and fermentation; Tequila

PMID:
23941710
DOI:
10.1016/j.biortech.2013.07.078
[Indexed for MEDLINE]

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