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Res Vet Sci. 2013 Dec;95(3):930-5. doi: 10.1016/j.rvsc.2013.07.015. Epub 2013 Aug 12.

Recombinant flagellin and its cross-talk with lipopolysaccharide--effect on pooled chicken peripheral blood mononuclear cells.

Author information

1
Recombinant DNA Laboratory, Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly 243 122, Uttar Pradesh, India. Electronic address: shishirgupta.biotech@gmail.com.

Abstract

Toll-like receptors (TLRs) are one of the types of pattern recognition receptors (PRRs) that recognize conserved pathogen molecules. TLRs link innate and adaptive arms of immune system and are implicated in the development of defense against invading pathogens. Lipopolysaccharide (LPS) and flagellin are recognized by TLR4 and TLR5, respectively. In this study, the effect of flagellin and lipopolysaccharide alone and in combination on chicken peripheral blood mononuclear cells (PBMCs) was investigated. The FliC gene of Salmonella typhimurium was expressed in a prokaryotic expression system and the recombinant flagellin was used to stimulate the chicken PBMCs. A combination of recombinant flagellin and LPS synergistically upregulated nitric oxide production, IL-12 and IL-6 expression but antagonistically down regulated IL-4 expression in comparison to recombinant flagellin alone. The results indicate that these agonists synergistically interact and enhance macrophage function and promote Th1 immune response in chicken PBMCs.

KEYWORDS:

Adjuvant; Agonist; Antagonism; Chicken PBMCs; Cross talk; Cytokine; Flagellin; Lipopolysaccharide; Synergism; Toll-like receptor

PMID:
23937992
DOI:
10.1016/j.rvsc.2013.07.015
[Indexed for MEDLINE]

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