Format

Send to

Choose Destination
See comment in PubMed Commons below
Anal Sci. 2013;29(8):811-4.

Simple preparation of green fluorescent protein conjugated with β-cyclodextrin in a site specific manner.

Author information

1
Department of Functional Materials and Science, Graduate School of Science and Engineering, Saitama University, 255 Shimo-okubo, Sakura, Saitama 338-8570, Japan. miho@fms.saitama-u.ac.jp

Abstract

We have site-directedly linked a green fluorescent protein (GFP) variant and a β-cyclodextrin (β-CD) with a simple method to develop a basic complex for sophisticated supramolecules. We have confirmed β-CD grafting on GFP with several methods including matrix-assisted laser desorption/ionization linear time-of-flight mass spectrometry (MALDI-TOF MS) without protease digestion and characterized the complex as well. In consideration of the resulting properties, the product we plainly and efficiently obtained could have applications related to sensing devices and drug delivery systems.

PMID:
23934562
[Indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for J-STAGE, Japan Science and Technology Information Aggregator, Electronic
    Loading ...
    Support Center