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J Chromatogr Sci. 2014 Sep;52(8):814-7. doi: 10.1093/chromsci/bmt120. Epub 2013 Aug 8.

Simultaneous quantitative determination of six active components in traditional Chinese medicinal preparation Cerebralcare Granule® by RP-HPLC coupled with diode array detection for quality control.

Author information

1
Tasly R&D Institute, Tianjin Tasly Group Co., Ltd., Tianjin 300410, China.
2
Tasly R&D Institute, Tianjin Tasly Group Co., Ltd., Tianjin 300410, China School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China maxiaohuitasly@sina.com.

Abstract

A simple, accurate and reliable method for the simultaneous separation and determination of six active components (protocatechuic acid, chlorogenic acid, caffeic acid, paeoniflorin, ferulic acid and rosmarinic acid) in traditional Chinese medicinal preparation Cerebralcare Granule(®) (CG) was developed using reverse-phase high-performance liquid chromatography coupled with diode array detector detection. The chromatographic separation was performed on a Hypersil GOLD C18 column with aqueous formic acid (0.1%, v/v) and acetonitrile as mobile phase at a flow rate of 0.2 ml/min at 30 °C. Because of the different UV characteristics of these components, change detection wavelength method was used for quantitative analysis. All of the analytes showed good linearity (r > 0.9992). The established method showed good precision and relative standard deviations (%) for intra-day and inter-day variations of 0.15-1.81 and 0.11-1.98%, respectively. The validated method was successfully applied to the simultaneously determination of six active components in CG from different batches.

PMID:
23934038
DOI:
10.1093/chromsci/bmt120
[Indexed for MEDLINE]

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