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J Pediatr Surg. 2013 Aug;48(8):1697-706. doi: 10.1016/j.jpedsurg.2012.11.038.

Protective effects of hydrogen-rich saline on necrotizing enterocolitis in neonatal rats.

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1
Department of General Surgery, Children's Hospital of Shanghai, Shanghai Jiaotong University School of Medicine, Shanghai 200040, P. R. China.

Abstract

PURPOSE:

The aim of this study was to test the hypothesis that hydrogen-rich saline (HRS) might have protective effects on the development of necrotizing enterocolitis (NEC) in a neonatal rat model.

METHODS:

NEC was induced in male newborn Sprague-Dawley rats by formula feeding, exposure to asphyxia and cold stress. Sixty-four rat pups were divided randomly into four groups: C+NS (n=11), C+H2 (n=11), NEC+NS (n=20), and NEC+H2 (n=22). Rats in the former two groups were mother-fed. Pups received intra-peritoneal injection of HRS (10 ml/kg, 10 min before asphyxia stress twice a day) or the same dose of normal saline. Rats were monitored until 96 h after birth. Body weight, histological NEC score, survival time, malondialdehyde, antioxidant capacity, inflammatory mediators, and mucosal integrity were assessed.

RESULTS:

HRS treatment maintained the body weight, reduced the incidence of NEC from 85% (17/20) to 54.5% (12/22), increased the survival rate from 25% (5/20) to 68.2% (15/22), and attenuated the severity of NEC. In addition, HRS inhibited the mRNA expression of pro-inflammatory mediators (inducible nitric oxide synthase, tumor necrosis factor-α, and interleukin-6), down-regulated lipid peroxidation, enhanced total antioxidant capacity, and prevented the increase of diamine oxidase in serum. However, no significant influence of HRS on the interleukin-10 mRNA expression was observed.

CONCLUSIONS:

HRS showed beneficial effects on neonatal rats with NEC via decreasing oxidative stress, increasing antioxidant capacity, suppressing inflammation, and preserving mucosal integrity.

KEYWORDS:

Hydrogen-rich saline; Inflammation; Necrotizing enterocolitis; Oxidative stress

PMID:
23932609
DOI:
10.1016/j.jpedsurg.2012.11.038
[Indexed for MEDLINE]
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