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Biotechniques. 2013 Aug;55(2):79-82. doi: 10.2144/000114065.

Mild hypothermia inhibits differentiation of human embryonic and induced pluripotent stem cells.

Author information

1
Department of Neuroscience, University of Connecticut Health Center, Farmington, CT, USA. belinsky@uchc.edu

Abstract

Culture of pluripotent stem cells at 35°C strikingly reduces unwanted spontaneous differentiation during hESC and iPSC maintenance compared with 37°C. Growth at 35°C did not affect expression of pluripotency mRNAs nor induce expression of cold-inducible genes. Colony size was somewhat reduced at 35°C. Thus, growth at 35°C is a convenient, simple method to reduce the labor of removing spontaneously differentiated colonies when maintaining pluripotent cells.

PMID:
23931596
DOI:
10.2144/000114065
[Indexed for MEDLINE]

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