Format

Send to

Choose Destination
Am J Transplant. 2013 Oct;13(10):2540-9. doi: 10.1111/ajt.12389. Epub 2013 Aug 8.

Increased T cell glucose uptake reflects acute rejection in lung grafts.

Author information

1
Division of Radiological Sciences and Nuclear Medicine, Department of Radiology, Washington University School of Medicine, St. Louis, MO.

Abstract

Although T cells are required for acute lung rejection, other graft-infiltrating cells such as neutrophils accumulate in allografts and are also high glucose utilizers. Positron emission tomography (PET) with the glucose probe [(18)F]fluorodeoxyglucose ([(18)F]FDG) has been employed to image solid organ acute rejection, but the sources of glucose utilization remain undefined. Using a mouse model of orthotopic lung transplantation, we analyzed glucose probe uptake in the grafts of syngeneic and allogeneic recipients with or without immunosuppression treatment. Pulmonary microPET scans demonstrated significantly higher [(18)F]FDG uptake in rejecting allografts when compared to transplanted lungs of either immunosuppressed or syngeneic recipients. [(18)F]FDG uptake was also markedly attenuated following T cell depletion therapy in lung recipients with ongoing acute rejection. Flow cytometric analysis using the fluorescent deoxyglucose analog 2-NBDG revealed that T cells, and in particular CD8(+) T cells, were the largest glucose utilizers in acutely rejecting lung grafts followed by neutrophils and antigen-presenting cells. These data indicate that imaging modalities tailored toward assessing T cell metabolism may be useful in identifying acute rejection in lung recipients.

KEYWORDS:

Allograft rejection; PET; T cell depletion; T lymphocyte activation; lung transplantation

PMID:
23927673
PMCID:
PMC3956601
DOI:
10.1111/ajt.12389
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center