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Anal Bioanal Chem. 2013 Oct;405(26):8475-86. doi: 10.1007/s00216-013-7268-4. Epub 2013 Aug 7.

Multiclass method for antimicrobial analysis in animal feeds by liquid chromatography-tandem mass spectrometry.

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Departament de Química Analítica, Universitat de Barcelona, 08028, Barcelona, Spain.


A rapid multiclass method that covers 50 antimicrobials from 13 different families in animal feeds was developed. Samples were extracted using a mixture of methanol, acetonitrile and a McIlvaine buffer combined with sonication. Feed extracts were simply diluted prior to injection, since the clean-up strategies that were tested, based on either solid-phase extraction or dispersive solid-phase extraction, were ineffective at minimizing matrix-related signal suppression/enhancement. Analysis was carried out by liquid chromatography coupled to tandem mass spectrometry using an electrospray ionization source operating in positive and negative modes. For the quantification, matrix-fortified standard calibration curves were used to compensate for matrix effects and losses in sample preparation. The method was validated in-house in pig, poultry and cattle feed matrices and showed satisfactory performance characteristics. Thus, the proposed approach was suitable for application in a routine high-throughput laboratory for the official control of feeds.

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