Hypotonic treatment leads to chromosomal aberrations but not to sister-chromatid exchanges in human lymphocytes

Mutat Res. 1990 Sep;245(1):5-9. doi: 10.1016/0165-7992(90)90017-e.

Abstract

Human peripheral lymphocytes were isolated from whole blood and exposed to culture medium of reduced osmolality. This hypotonic treatment led to a significant increase in the frequencies of chromosomal aberrations when the osmolality was reduced to 60 mOsm/kg H2O and below. Maximum damage occurred when the hypotonic treatment was done 27 or 30 h after starting the cultures. We also looked for the induction of sister-chromatid exchanges (SCE) by hypotonic culture conditions, but the SCE frequencies were not influenced.

MeSH terms

  • Cell Cycle
  • Chromosome Aberrations / genetics*
  • Culture Media
  • Humans
  • Hydrogen-Ion Concentration
  • Lymphocytes / ultrastructure*
  • Mitotic Index
  • Osmolar Concentration
  • Sister Chromatid Exchange / genetics*

Substances

  • Culture Media