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Carbohydr Res. 2013 Oct 18;380:45-50. doi: 10.1016/j.carres.2013.07.005. Epub 2013 Jul 20.

The novel structure of the core oligosaccharide backbone of the lipopolysaccharide from the Plesiomonas shigelloides strain CNCTC 80/89 (serotype O13).

Author information

1
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, PL-53-114 Wroclaw, Poland. Electronic address: Marta.Kaszowska@iitd.pan.wroc.pl.

Abstract

The new structure of the core oligosaccharide of Plesiomonas shigelloides CNCTC 80/89 (serotype O13) lipopolysaccharide has been investigated by chemical methods, (1)H and (13)C NMR spectroscopy and matrix-assisted laser-desorption/ionization time of flight (MALDI-TOF). It was concluded that the core oligosaccharide of P. shigelloides CNCTC 80/89 is a nonasaccharide with the following structure: The position of glycine was determined by MALDI-TOF MS/MS analyses.

KEYWORDS:

CNCTC; COSY; Core oligosaccharide; Czech National Collection of Type Cultures; DEPT; GC; Glycine; HMBC; HSQC; LPS; Lipopolysaccharide; MALDI-TOF; MALDI-TOF MS/MS; MS; NMR; NOESY; OS; Plesiomonas shigelloides; TOCSY; correlated spectroscopy; distortionless enhancement by polarization transfer; gas chromatography; heteronuclear multiple bond correlation; heteronuclear single quantum coherence; lipopolysaccharide; mass spectrometry; matrix-assisted laser-desorption/ionization time-of-flight; nuclear Overhauser effect spectroscopy; nuclear magnetic resonance; oligosaccharide; total correlation spectroscopy

PMID:
23920477
DOI:
10.1016/j.carres.2013.07.005
[Indexed for MEDLINE]

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