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Gastroenterology. 2013 Nov;145(5):1026-34. doi: 10.1053/j.gastro.2013.07.044. Epub 2013 Jul 31.

The hepatitis B vaccine protects re-exposed health care workers, but does not provide sterilizing immunity.

Author information

1
Immunology Section, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland; Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland.

Abstract

BACKGROUND & AIMS:

Infection with hepatitis B virus (HBV) can be prevented by vaccination with HB surface (HBs) antigen, which induces HBs-specific antibodies and T cells. However, the duration of vaccine-induced protective immunity is poorly defined for health care workers who were vaccinated as adults.

METHODS:

We investigated the immune mechanisms (antibody and T-cell responses) of long-term protection by the HBV vaccine in 90 health care workers with or without occupational exposure to HBV, 10-28 years after vaccination.

RESULTS:

Fifty-nine of 90 health care workers (65%) had levels of antibodies to HBs antigen above the cut-off (>12 mIU/mL) and 30 of 90 (33%) had HBs-specific T cells that produced interferon-gamma. Titers of antibodies to HBs antigen correlated with numbers of HBs-specific interferon-gamma-producing T cells, but not with time after vaccination. Although occupational exposure to HBV after vaccination did not induce antibodies to the HBV core protein (HBcore), the standard biomarker for HBV infection, CD4(+) and CD8(+) T cells against HBcore and polymerase antigens were detected. Similar numbers of HBcore- and polymerase-specific CD4(+) and CD8(+) T cells were detected in health care workers with occupational exposure to HBV and in patients who acquired immunity via HBV infection. Most of the HBcore- and polymerase-specific T cells were CD45RO(+)CCR7(-)CD127(-) effector memory cells in exposed health care workers and in patients with acquired immunity. In contrast, most of the vaccine-induced HBs-specific T cells were CD45RO(-)CCR7(-)CD127(-) terminally differentiated cells.

CONCLUSIONS:

HBs antigen vaccine-induced immunity protects against future infection but does not provide sterilizing immunity, as evidenced by HBcore- and polymerase-specific CD8(+) T cells in vaccinated health care workers with occupational exposure to HBV. The presence of HBcore- and HBV polymerase-specific T-cell responses is a more sensitive indicator of HBV exposure than detection of HBcore-specific antibodies.

KEYWORDS:

DMSO; FCS; HBV; HBV core protein; HBV polymerase; HBVpol; HBcore; HBsAg; HDV; IFN; Immune Response; Immunization; PBMC; PBS; PE; PHA-M; T Cell; Virus; anti-HBs; antibody to hepatitis B surface antigen; dimethyl sulfoxide; fetal calf serum; hepatitis B surface antigen; hepatitis B virus; hepatitis D virus; interferon; peripheral blood mononuclear cells; phosphate-buffered saline; phycoerythrin; phytohemagglutinin

PMID:
23916846
PMCID:
PMC3884684
DOI:
10.1053/j.gastro.2013.07.044
[Indexed for MEDLINE]
Free PMC Article

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