Send to

Choose Destination
J Mech Behav Biomed Mater. 2013 Nov;27:115-27. doi: 10.1016/j.jmbbm.2013.07.008. Epub 2013 Jul 16.

Characterizing the micro-scale elastic modulus of hydrogels for use in regenerative medicine.

Author information

Wake Forest Institute for Regenerative Medicine, Richard H. Dean Biomedical Research Building, 391 Technology Way, Winston-Salem, NC 27101, United States. Electronic address:


Our objective was to characterize the elasticity of hydrogel formulations intended to mimic physical properties that cells and tissues experience in vivo. Using atomic force microscopy (AFM), we tested a variety of concentrations in a variety of biomaterials, including agarose, alginate, the collagens, fibrin, hyaluronic acid, kerateine, laminin, Matrigel, polyacrylamide, polyethylene glycol diacrylate (PEGDA) and silicone elastomer (polydimethylsiloxane). Manipulations of the concentration of biomaterials were detectable in AFM measurements of elasticity (Young's modulus, E), and E tended to increase with increased concentration. Depending on the biomaterials chosen, and their concentrations, generation of tunable biocompatible hydrogels in the physiologic range is possible.


Atomic force microscopy (AFM); Biomaterials; Elastic modulus; Hydrogel; Substrates; Tissue engineering

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center