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Genes Dev. 2013 Aug 1;27(15):1656-61. doi: 10.1101/gad.221515.113.

DmGTSF1 is necessary for Piwi-piRISC-mediated transcriptional transposon silencing in the Drosophila ovary.

Author information

1
Department of Molecular Biology, Keio University School of Medicine, Tokyo 160-8582, Japan

Abstract

The Piwi-piRNA (PIWI-interacting RNA) complex (Piwi-piRISC) in Drosophila ovarian somatic cells represses transposons transcriptionally to maintain genome integrity; however, the underlying mechanisms remain obscure. Here, we reveal that DmGTSF1, a Drosophila homolog of gametocyte-specific factor 1 (GTSF1) (which is required for transposon silencing in mouse testes), is necessary for Piwi-piRISC to repress target transposons and neighboring genes. DmGTSF1 depletion affected neither piRNA biogenesis nor nuclear import of Piwi-piRISC. DmGTSF1 mutations caused derepression of transposons and loss of ovary follicle layers, resulting in female infertility. We suggest that DmGTSF1, a nuclear Piwi interactor, is an integral factor in Piwi-piRISC-mediated transcriptional silencing.

KEYWORDS:

DmGTSF1; Drosophila; Piwi; RNA silencing; piRNA; transposon

PMID:
23913921
PMCID:
PMC3744724
DOI:
10.1101/gad.221515.113
[Indexed for MEDLINE]
Free PMC Article

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