Hydrogel micropillars with integrin selective peptidomimetic functionalized nanopatterned tops: a new tool for the measurement of cell traction forces transmitted through αvβ3- or α5β1-integrins

Adv Mater. 2013 Nov 6;25(41):5869-74. doi: 10.1002/adma.201301338. Epub 2013 Aug 5.

Abstract

Poly(ethylene glycol) micropillars with gold nanopatterns on top are functionalized with two integrin selective ligands. This platform is a powerful new tool to determine the specific contribution of traction forces involved in cell adhesion mediated by α5β1- and αvβ3-integrins. Cells adherent via α5β1-integrins have a tendency to exert higher maximum forces than cells adhering via αvβ3-integrins.

Keywords: cell adhesion; integrin; micropillar; peptidomimetics; traction force.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomechanical Phenomena
  • Gold / chemistry
  • Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry
  • Integrin alpha5beta1 / metabolism*
  • Integrin alphaVbeta3 / metabolism*
  • Mechanical Phenomena*
  • Metal Nanoparticles / chemistry*
  • Micelles
  • Microscopy*
  • Peptidomimetics / chemistry*
  • Peptidomimetics / metabolism*
  • Polyethylene Glycols / chemistry*
  • Substrate Specificity

Substances

  • Integrin alpha5beta1
  • Integrin alphaVbeta3
  • Micelles
  • Peptidomimetics
  • Hydrogel, Polyethylene Glycol Dimethacrylate
  • Polyethylene Glycols
  • Gold