Abstract
Poly(ethylene glycol) micropillars with gold nanopatterns on top are functionalized with two integrin selective ligands. This platform is a powerful new tool to determine the specific contribution of traction forces involved in cell adhesion mediated by α5β1- and αvβ3-integrins. Cells adherent via α5β1-integrins have a tendency to exert higher maximum forces than cells adhering via αvβ3-integrins.
Keywords:
cell adhesion; integrin; micropillar; peptidomimetics; traction force.
© 2013 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biomechanical Phenomena
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Gold / chemistry
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Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry
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Integrin alpha5beta1 / metabolism*
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Integrin alphaVbeta3 / metabolism*
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Mechanical Phenomena*
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Metal Nanoparticles / chemistry*
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Micelles
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Microscopy*
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Peptidomimetics / chemistry*
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Peptidomimetics / metabolism*
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Polyethylene Glycols / chemistry*
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Substrate Specificity
Substances
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Integrin alpha5beta1
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Integrin alphaVbeta3
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Micelles
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Peptidomimetics
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Hydrogel, Polyethylene Glycol Dimethacrylate
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Polyethylene Glycols
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Gold