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Nat Methods. 2013 Sep;10(9):861-4. doi: 10.1038/nmeth.2596. Epub 2013 Aug 4.

3D spectral imaging with synchrotron Fourier transform infrared spectro-microtomography.

Author information

1
Advanced Light Source Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA. MCMartin@lbl.gov

Abstract

We report Fourier transform infrared spectro-microtomography, a nondestructive three-dimensional imaging approach that reveals the distribution of distinctive chemical compositions throughout an intact biological or materials sample. The method combines mid-infrared absorption contrast with computed tomographic data acquisition and reconstruction to enhance chemical and morphological localization by determining a complete infrared spectrum for every voxel (millions of spectra determined per sample).

PMID:
23913258
DOI:
10.1038/nmeth.2596
[Indexed for MEDLINE]

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