Format

Send to

Choose Destination
See comment in PubMed Commons below
Arch Microbiol. 2013 Sep;195(9):655-60. doi: 10.1007/s00203-013-0917-3. Epub 2013 Aug 4.

Enterocin T, a novel class IIa bacteriocin produced by Enterococcus sp. 812.

Author information

1
Department of Biotechnology, Ming Chuan University, No. 5 De-Ming Rd., Gui-Shan Township, Taoyuan, 333, Taiwan. yisheng@mail.mcu.edu.tw

Abstract

Enterococcus sp. 812, isolated from fresh broccoli, was previously found to produce a bacteriocin active against a number of Gram-positive bacteria, including Listeria monocytogenes. Bacteriocin activity decreased slightly after autoclaving (121 °C for 15 min), but was inactivated by protease K. Mass spectrometry analysis revealed the bacteriocin mass to be approximately 4,521.34 Da. N-terminal amino acid sequencing yielded a partial sequence, NH2-ATYYGNGVYXDKKKXWVEWGQA, by Edman degradation, which contained the consensus class IIa bacteriocin motif YGNGV in the N-terminal region. The obtained partial sequence showed high homology with some enterococcal bacteriocins; however, no identical peptide or protein was found. This peptide was therefore considered to be a novel bacteriocin produced by Enterococcus sp. 812 and was termed enterocin T.

PMID:
23912616
DOI:
10.1007/s00203-013-0917-3
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer
    Loading ...
    Support Center