Format

Send to

Choose Destination
J Biol Chem. 2013 Sep 20;288(38):27287-98. doi: 10.1074/jbc.M113.498899. Epub 2013 Aug 1.

Double-membraned liposomes sculpted by poliovirus 3AB protein.

Author information

1
From the Department of Physiology and Biophysics, Boston University School of Medicine, Boston, Massachusetts 02118 and.

Abstract

Infection with many positive-strand RNA viruses dramatically remodels cellular membranes, resulting in the accumulation of double-membraned vesicles that resemble cellular autophagosomes. In this study, a single protein encoded by poliovirus, 3AB, is shown to be sufficient to induce the formation of double-membraned liposomes via the invagination of single-membraned liposomes. Poliovirus 3AB is a 109-amino acid protein with a natively unstructured N-terminal domain. HeLa cells transduced with 3AB protein displayed intracellular membrane disruption; specifically, the formation of cytoplasmic invaginations. The ability of a single viral protein to produce structures of similar topology to cellular autophagosomes should facilitate the understanding of both cellular and viral mechanisms for membrane remodeling.

KEYWORDS:

Lipoprotein; Liposomes; Membrane Bilayer; Membrane Biophysics; Membrane Reconstitution; Positive-strand RNA Viruses; Viral Protein; Viral Replication

PMID:
23908350
PMCID:
PMC3779724
DOI:
10.1074/jbc.M113.498899
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center