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Plant Cell. 2013 Jun;25(6):2171-86. doi: 10.1105/tpc.113.113043. Epub 2013 Jun 28.

The caspase-related protease separase (extra spindle poles) regulates cell polarity and cytokinesis in Arabidopsis.

Author information

1
Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linean Center for Plant Biology, SE-75007 Uppsala, Sweden. panagiotis.moschou@slu.se

Erratum in

  • Plant Cell. 2014 Sep;26(9):3823.

Abstract

Vesicle trafficking plays an important role in cell division, establishment of cell polarity, and translation of environmental cues to developmental responses. However, the molecular mechanisms regulating vesicle trafficking remain poorly understood. Here, we report that the evolutionarily conserved caspase-related protease separase (extra spindle poles [ESP]) is required for the establishment of cell polarity and cytokinesis in Arabidopsis thaliana. At the cellular level, separase colocalizes with microtubules and RabA2a (for RAS genes from rat brainA2a) GTPase-positive structures. Separase facilitates polar targeting of the auxin efflux carrier PIN-formed2 (PIN2) to the rootward side of the root cortex cells. Plants with the radially swollen4 (rsw4) allele with compromised separase activity, in addition to mitotic failure, display isotropic cell growth, perturbation of auxin gradient formation, slower gravitropic response in roots, and cytokinetic failure. Measurements of the dynamics of vesicle markers on the cell plate revealed an overall reduction of the delivery rates of KNOLLE and RabA2a GTPase in separase-deficient roots. Furthermore, dissociation of the clathrin light chain, a protein that plays major role in the formation of coated vesicles, was slower in rsw4 than in the control. Our results demonstrate that separase is a key regulator of vesicle trafficking, which is indispensable for cytokinesis and the establishment of cell polarity.

PMID:
23898031
PMCID:
PMC3723619
DOI:
10.1105/tpc.113.113043
[Indexed for MEDLINE]
Free PMC Article
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