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Mycoses. 2014 Mar;57(3):135-40. doi: 10.1111/myc.12115. Epub 2013 Jul 29.

MALDI-TOF mass spectrometry identification of filamentous fungi in the clinical laboratory.

Author information

1
UMR MD3, Aix-Marseille Université, Marseille, France; Laboratoire de Parasitologie-Mycologie, APHM Timone, Marseille, France.

Abstract

This study aimed to validate the effectiveness of a standardised procedure for the MALDI-TOF mass spectrometry (MS)-based identification on a large sample of filamentous fungi routinely identified in university hospitals' laboratories. Non-dermatophyte filamentous fungi prospectively isolated in the routine activity of five teaching hospitals in France were first identified by conventional methods in each laboratory and then by MS in one centre. DNA sequence-based identification resolved discrepancies between both methods. In this study, of the 625 analysed filamentous fungi of 58 species, 501 (80%) and 556 (89%) were correctly identified by conventional methods and MS respectively. Compared with the conventional method, MS dramatically enhanced the performance of the identification of the non-Aspergillus filamentous fungi with a 31-61% increase in correct identification rate. In conclusion, this study on a large sample of clinical filamentous fungi taxa demonstrates that species identification is significantly improved by MS compared with the conventional method. The main limitation is that MS identification is possible only if the species is included in the reference spectra library. Nevertheless, for the routine clinical laboratory, MS provides the means to attain markedly accurate results in filamentous fungi identification, which was previously restricted to only a few reference laboratories.

KEYWORDS:

MALDI-TOF; filamentous fungi; identification; routine laboratory

PMID:
23895114
DOI:
10.1111/myc.12115
[Indexed for MEDLINE]

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