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PLoS One. 2013 Jul 23;8(7):e68824. doi: 10.1371/journal.pone.0068824. Print 2013.

Advantages of Single-Molecule Real-Time Sequencing in High-GC Content Genomes.

Author information

1
Korea Polar Research Institute, Yeonsu-gu, Incheon, Korea.

Abstract

Next-generation sequencing has become the most widely used sequencing technology in genomics research, but it has inherent drawbacks when dealing with high-GC content genomes. Recently, single-molecule real-time sequencing technology (SMRT) was introduced as a third-generation sequencing strategy to compensate for this drawback. Here, we report that the unbiased and longer read length of SMRT sequencing markedly improved genome assembly with high GC content via gap filling and repeat resolution.

PMID:
23894349
PMCID:
PMC3720884
DOI:
10.1371/journal.pone.0068824
[Indexed for MEDLINE]
Free PMC Article

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