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J Clin Pathol. 2013 Dec;66(12):1051-7. doi: 10.1136/jclinpath-2012-201391. Epub 2013 Jul 26.

EP1: a novel rabbit monoclonal antibody for detection of oestrogen receptor α.

Author information

1
Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, , Indianapolis, Indiana, USA.

Abstract

AIMS:

Assessment of hormone receptor expression is part of routine examination of every breast cancer. In this study, we report the characterisation of a novel rabbit monoclonal antibody, clone EP1, directed against oestrogen receptor (ER) α. Additionally, its immunohistochemical performance characteristics in archival tissues are evaluated in normal tissues and two distinct cohorts of breast cancer patients.

METHODS:

Comparative analyses between EP1 and the anti-ERα component of the ER/PR pharmDx kit (cocktail of mouse monoclonal antibody clones 1D5 and ER-2-123) and between EP1 and another commercially available rabbit monoclonal antibody, clone SP1, are described.

RESULTS:

Clone EP1 specifically detects nuclear ER in all tissues examined; cytoplasmic staining was not observed. The analysis shows a high degree of concordance (~95%) between EP1 and both the ERα component of the Dako ER/PR pharmDx kit and Ventana clone SP1. However, the use of EP1 antibody together with Dako EnVision FLEX detection system resulted in a stronger staining intensity as compared with SP1 antibody using the Ventana ultraView DAB detection system resulting in better 'ease of use.'

CONCLUSIONS:

The use of EPI can result in better interpretation of the results of the ER analysis.

KEYWORDS:

Antibodies; Breast Cancer; Immunohistochemistry

PMID:
23894168
PMCID:
PMC3841770
DOI:
10.1136/jclinpath-2012-201391
[Indexed for MEDLINE]
Free PMC Article

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