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Biosens Bioelectron. 2013 Dec 15;50:406-13. doi: 10.1016/j.bios.2013.07.006. Epub 2013 Jul 11.

Magnesium oxide grafted carbon nanotubes based impedimetric genosensor for biomedical application.

Author information

1
Department of Science and Technology Centre on Biomolecular Electronics, Biomedical Instrumentation Section, CSIR-National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi 110012, India; Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi 110025, India.

Abstract

Nanostructured magnesium oxide (size<10nm) grafted carboxyl (COOH) functionalized multi-walled carbon nanotubes (nMgO-cMWCNTs) deposited electrophoretically onto indium tin oxide (ITO) coated glass electrode and have been utilized for Vibrio cholerae detection. Aminated 23 bases single stranded DNA (NH2-ssDNA) probe sequence (O1 gene) of V. cholerae has been covalently functionalized onto nMgO-cMWCNTs/ITO electrode surface using EDC-NHS chemistry. This DNA functionalized MgO grafted cMWCNTs electrode has been characterized using X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and electrochemical techniques. The results of XPS studies reveal that sufficient O-C=O groups present at the nMgO-cMWCNTs surface are utilized for DNA binding. The results of hybridization studies conducted with fragmented target DNA (ftDNA) of V. cholerae using electrochemical impedance spectroscopy (EIS) reveal sensitivity as 3.87 Ω ng(-1) cm(-2), detection limit of ~21.70 ng µL(-1) in the linear range of 100-500 ng µL(-1) and stability of about 120 days. The proposed DNA functionalized nMgO-cMWCNTs nanomatrix provides a novel impedimetric platform for the fabrication of a compact genosensor device for biomedical application.

KEYWORDS:

DNA functionalization; Genosensor; Impedance spectroscopy; Magnesium oxide; Multi-walled carbon nanotubes; Vibrio cholerae

PMID:
23893063
DOI:
10.1016/j.bios.2013.07.006
[Indexed for MEDLINE]

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