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Nat Methods. 2013 Sep;10(9):903-9. doi: 10.1038/nmeth.2572. Epub 2013 Jul 28.

Rapid and accurate large-scale genotyping of duplicated genes and discovery of interlocus gene conversions.

Author information

1
Department of Genome Sciences, University of Washington School of Medicine, Seattle, Washington, USA.

Abstract

Over 900 genes have been annotated within duplicated regions of the human genome, yet their functions and potential roles in disease remain largely unknown. One major obstacle has been the inability to accurately and comprehensively assay genetic variation for these genes in a high-throughput manner. We developed a sequencing-based method for rapid and high-throughput genotyping of duplicated genes using molecular inversion probes designed to target unique paralogous sequence variants. We applied this method to genotype all members of two gene families, SRGAP2 and RH, among a diversity panel of 1,056 humans. The approach could accurately distinguish copy number in paralogs having up to ∼99.6% sequence identity, identify small gene-disruptive deletions, detect single-nucleotide variants, define breakpoints of unequal crossover and discover regions of interlocus gene conversion. The ability to rapidly and accurately genotype multiple gene families in thousands of individuals at low cost enables the development of genome-wide gene conversion maps and 'unlocks' many previously inaccessible duplicated genes for association with human traits.

PMID:
23892896
PMCID:
PMC3985568
DOI:
10.1038/nmeth.2572
[Indexed for MEDLINE]
Free PMC Article

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