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Nat Methods. 2013 Oct;10(10):973-6. doi: 10.1038/nmeth.2600. Epub 2013 Jul 25.

RNA-guided gene activation by CRISPR-Cas9-based transcription factors.

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1
Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA.

Abstract

Technologies for engineering synthetic transcription factors have enabled many advances in medical and scientific research. In contrast to existing methods based on engineering of DNA-binding proteins, we created a Cas9-based transactivator that is targeted to DNA sequences by guide RNA molecules. Coexpression of this transactivator and combinations of guide RNAs in human cells induced specific expression of endogenous target genes, demonstrating a simple and versatile approach for RNA-guided gene activation.

PMID:
23892895
PMCID:
PMC3911785
DOI:
10.1038/nmeth.2600
[Indexed for MEDLINE]
Free PMC Article
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