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FEBS Lett. 2013 Sep 17;587(18):3032-7. doi: 10.1016/j.febslet.2013.07.033. Epub 2013 Jul 24.

LMO4 modulates proliferation and differentiation of 3T3-L1 preadipocytes.

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1
College of Animal Science and Technology, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, Heilongjiang 150030, China. wangning@neau.edu.cn

Abstract

Previous microarray analyses revealed that LMO4 is expressed in 3T3-L1 preadipocytes, however, its roles in adipogenesis are unknown. In the present study, using RT-PCR sequencing and quantitative real-time RT-PCR, we confirmed that LMO4 gene is expressed in 3T3-L1 preadipocytes and its expression peaks at the early stage of 3T3-L1 preadipocyte differentiation. Further analyses showed that LMO4 knockdown decreased the proliferation of 3T3-L1 preadipocytes, and attenuated the differentiation of 3T3-L1 preadipocytes, as evidenced by reduced lipid accumulation and down-regulation of PPARγ gene expression. Collectively, our findings indicate that LMO4 is a novel modulator of adipogenesis.

KEYWORDS:

3T3-L1 preadipocyte; Cell proliferation; LMO4; Preadipocyte differentiation

PMID:
23892074
DOI:
10.1016/j.febslet.2013.07.033
[Indexed for MEDLINE]
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