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Mitochondrion. 2013 Nov;13(6):871-80. doi: 10.1016/j.mito.2013.07.115. Epub 2013 Jul 24.

Interactions between peptidyl tRNA hydrolase homologs and the ribosomal release factor Mrf1 in S. pombe mitochondria.

Author information

1
Centre de Génétique Moléculaire, UPR3404, FRC3115, Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France.

Abstract

Mitochondrial translation synthesizes key subunits of the respiratory complexes. In Schizosaccharomyces pombe, strains lacking Mrf1, the mitochondrial stop codon recognition factor, are viable, suggesting that other factors can play a role in translation termination. S. pombe contains four predicted peptidyl tRNA hydrolases, two of which (Pth3 and Pth4), have a GGQ motif that is conserved in class I release factors. We show that high dosage of Pth4 can compensate for the absence of Mrf1 and loss of Pth4 exacerbates the lack of Mrf1. Also Pth4 is a component of the mitochondrial ribosome, suggesting that it could help recycling stalled ribosomes.

KEYWORDS:

Fission yeast; Mitochondria; Mitochondrial ribosome tagging; Peptidyl tRNA hydrolase; Release factor; Translation

PMID:
23892058
PMCID:
PMC3919214
DOI:
10.1016/j.mito.2013.07.115
[Indexed for MEDLINE]
Free PMC Article

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