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Biochem Biophys Res Commun. 2013 Aug 23;438(2):289-94. doi: 10.1016/j.bbrc.2013.07.057. Epub 2013 Jul 23.

High-level heterologous expression of fungal cytochrome P450s in Escherichia coli.

Author information

1
Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. ichinose@agr.kyushu-u.ac.jp

Abstract

A thorough understanding of the sequence-structure-function relationships of cytochrome P450 (P450) is necessary to better understand the metabolic diversity of living organisms. Significant amounts of pure enzymes are sometimes required for biochemical studies, and their acquisition often relies on the possibility of their heterologous expression. In this study, we performed extensive heterologous expression of fungal P450s in Escherichia coli using 304 P450 isoforms. Using large-scale screening, we confirmed that at least 27 P450s could be expressed with/without simple sequence deletion at the 5' end of cDNAs, which encode the N-terminal hydrophobic domain of the enzyme. Moreover, we identified N-terminal amino acid sequences that can potentially be used to construct chimeric P450s, which could dramatically improve their expression levels even when the expression of the wild-type sequence was unpromising. These findings will help increase the chance of heterologous expression of a variety of fungal and other eukaryotic membrane-bound P450s in E. coli.

KEYWORDS:

2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid; EDTA; Escherichia coli; Eukaryotic cytochrome P450; HEPES; Heterologous expression; High-level expression; IPTG; Large-scale screening; P450; PMSF; TMD; Wood-rotting basidiomycetes; cytochrome P450 monooxygenase; ethylenediaminetetraacetic acid; isopropyl-1-thio-β-d-galactopyranoside; phenylmethylsulfonyl fluoride; transmembrane domain

PMID:
23886957
DOI:
10.1016/j.bbrc.2013.07.057
[Indexed for MEDLINE]
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