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Zhonghua Yi Xue Za Zhi. 2013 Apr 2;93(13):1028-31.

[RNAi-mediated knockdown of cyclooxygenase 2 inhibits the growth and migration of SaOS2 human osteosarcoma cells].

[Article in Chinese]

Author information

1
Department of Orthopedics, Fuyang Cancer Hospital, Anhui 236000, China.

Abstract

OBJECTIVE:

To explore the effects of a knockdown of cyclooxygenase 2 upon the growth and migration of SaOS2 cells.

METHODS:

We employed lentivirus mediated-RNA interference technology to knockdown the endogenous expression of gene COX-2 in human osteosarcoma cells (SaOS2) and analyzed their phenotypical changes. The effects of COX-2 silencing on the proliferation, cell cycle and migration of SaOS2 cells were assessed by thiazolyl blue tetrazolium bromide, flow cytometry and migration assays respectively. COX-2, vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), basic fibroblast growth factor (bFGF) mRNA and protein expression were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot.

RESULTS:

A decreased expression of COX-2 in human osteosarcoma cells significantly inhibited the growth and decreased the migration ability of SaOS2 cells. Furthermore, it also reduced VEGF, EGF and bFGF mRNA and protein expression.

CONCLUSION:

The COX-2 signaling pathway may provide a novel therapeutic target for the treatment of human osteosarcoma.

PMID:
23886271
[Indexed for MEDLINE]
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