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Arch Virol. 2014 Jan;159(1):147-51. doi: 10.1007/s00705-013-1793-5. Epub 2013 Jul 25.

Natural antisense transcripts of UL123 packaged in human cytomegalovirus virions.

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1
State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.

Abstract

In this study, we demonstrated that antisense transcripts of human cytomegalovirus (HCMV) UL123, UL21.5 and cellular GAPDH genes were present in highly purified virions. These virion RNAs were delivered into the host cells upon infection, and de novo synthesized ones appeared in the infected cell at the immediate early stage. Although the sequence of UL123 antisense transcripts in virions is uncertain, we found that these transcripts in Towne-infected human fibroblasts had novel transcriptional start sites (TSSs) with various 5'-terminal deletions of open reading frame (ORF) 59. These findings not only provide new insight into the composition of HCMV virions but also reveal a possible viral strategy for initiating latent infection and switching between latent and productive infections.

PMID:
23884634
PMCID:
PMC5672920
DOI:
10.1007/s00705-013-1793-5
[Indexed for MEDLINE]
Free PMC Article
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