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J Pediatr Gastroenterol Nutr. 2013 Aug;57(2):192-6. doi: 10.1097/MPG.0b013e318293e1e1.

Use of the noninvasive entero-test in the detection of Helicobacter pylori in children in an endemic area in Colombia.

Author information

1
Department of Medicine, Division of Gastroenterology, Vanderbilt University School of Medicine, Nashville, TN 37232 , USA.

Abstract

BACKGROUND AND OBJECTIVE:

Gastric infection with Helicobacter pylori (H pylori), a strong risk factor for gastric cancer, is highly prevalent in children residing in the Colombian Andes. We aimed to validate the use of the Entero-test to culture and genotype H pylori strains from asymptomatic Colombian children.

METHODS:

Children (ages 10-15 years, n = 110, 80 of which were H pylori positive by the urea breath test [UBT]) were subjected to the Entero-test, and strings were cultured and/or used for DNA extraction for polymerase chain reaction (PCR). These children had been treated for H pylori in 2007. A second population of children (ages 10-15 years, n = 95),which had not been previously treated, was also subjected to the Entero-test.

RESULTS:

Of UBT-positive children in the treated group, 29 of 80 (36%) Entero-test samples were H pylori culture positive; 29 additional string extracts were tested by PCR for the H pylori virulence factors cagA and vacA. PCR from cultures and extracts yielded a sensitivity of 74% and specificity of 87%. In the untreated group, 16 of 94 UBT-positive children (17%) produced Entero-tests that were culture positive. Fifty-eight of 94 (62%) string extracts were PCR positive for cagA and/or vacA. In previously treated children, H pylori strains were more often the less virulent vacA s2 (P = 0.001), m2 (P = 0.006), and i2 genotypes (P = 0.039).

CONCLUSIONS:

The Entero-test may be used as a noninvasive test to detect H pylori in asymptomatic children residing in high-risk areas for gastric cancer. Treatment of H pylori in children was associated with less virulent genotypes.

PMID:
23880626
PMCID:
PMC3725653
DOI:
10.1097/MPG.0b013e318293e1e1
[Indexed for MEDLINE]
Free PMC Article

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