Format

Send to

Choose Destination
See comment in PubMed Commons below
Pathol Biol (Paris). 2013 Dec;61(6):280-1. doi: 10.1016/j.patbio.2013.05.006. Epub 2013 Jul 20.

Staphylococcal cassette chromosome mec characterization of methicillin-resistant Staphylococcus aureus strains isolated at the military hospital of Constantine/Algeria.

Author information

1
Unité de microbiologie, hôpital militaire universitaire de Constantine, Nouvelle ville Ali Mendjeli, Constantine, Algeria. Electronic address: z_ouche@yahoo.fr.

Abstract

Staphylococcal cassette chromosome mec is a genetic mobile element that carries the gene mecA mediating the methicillin resistance in staphylococci. The aim of this study is to type the Staphylococcal cassette chromosome mec (SCCmec) in 64 non-redundant methicillin-resistant Staphylococcus aureus (MRSA) strains recovered at the military hospital of Constantine (Algeria) between 2005 and 2007. Methicillin resistance was detected by oxacillin and cefoxitin discs and PBP2a test, and then confirmed by mecA PCR. The SCCmec complex types were determined by real time PCR. The analysis showed that 50 isolates were hospital acquired (HA-MRSA) and 14 were community-acquired (CA-MRSA). SCCmec type IV and V (traditionally attributed to CA-MRSA) were harbored by both HA-MRSA and CA-MRSA, while SCCmec type I, II and III were not recorded. These findings motivate more investigations to be carried on HA-MRSA in our hospital and other national health care centers.

KEYWORDS:

Algeria; Algérie; MRSA; SARM; SCCmec; Staphylococcus aureus

PMID:
23880229
DOI:
10.1016/j.patbio.2013.05.006
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center