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Biochemistry. 2013 Aug 6;52(31):5155-7. doi: 10.1021/bi4005655. Epub 2013 Jul 24.

Conclusive evidence of the reconstituted hexasome proven by native mass spectrometry.

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1
Graduate School of Nanobioscience, Yokohama City University , 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.

Abstract

It has been suggested that the hexasome, in which one of the H2A/H2B dimers is depleted from the canonical nucleosome core particle (NCP), is an essential intermediate during NCP assembly and disassembly, but little structural evidence of this exists. In this study, reconstituted products in a conventional NCP preparation were analyzed by native electrospray ionization mass spectrometry, and it was found that the hexasome, which migrated in a manner almost identical to that of the octasome NCP in native polyacrylamide gel electrophoresis, was produced simultaneously with the octasome NCP. This result might contribute to understanding the assembly and disassembly mechanism of NCPs.

PMID:
23879667
DOI:
10.1021/bi4005655
[Indexed for MEDLINE]
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