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Genes Dev. 2013 Jul 15;27(14):1596-609. doi: 10.1101/gad.216192.113.

A TAF4 coactivator function for E proteins that involves enhanced TFIID binding.

Author information

1
Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, New York 10065, USA.

Abstract

The multisubunit TFIID plays a direct role in transcription initiation by binding to core promoter elements and directing preinitiation complex assembly. Although TFIID may also function as a coactivator through direct interactions with promoter-bound activators, mechanistic aspects of this poorly defined function remain unclear. Here, biochemical studies show a direct TFIID-E-protein interaction that (1) is mediated through interaction of a novel E-protein activation domain (activation domain 3 [AD3]) with the TAF homology (TAFH) domain of TAF4, (2) is critical for activation of a natural target gene by an E protein, and (3) mechanistically acts by enhancing TFIID binding to the core promoter. Complementary assays establish a gene-specific role for the TAFH domain in TFIID recruitment and activation of a large subset of genes in vivo. These results firmly establish TAF4 as a bona fide E-protein coactivator as well as a mechanism involving facilitated TFIID binding through direct interaction with an E-protein activation domain.

KEYWORDS:

E proteins; E2A; HEB; TAF4; TAFH; TFIID; transcription coactivator

PMID:
23873942
PMCID:
PMC3731548
DOI:
10.1101/gad.216192.113
[Indexed for MEDLINE]
Free PMC Article
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