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Genes Dev. 2013 Jul 15;27(14):1545-50. doi: 10.1101/gad.221648.113.

Subnuclear partitioning of rRNA genes between the nucleolus and nucleoplasm reflects alternative epiallelic states.

Author information

1
Department of Biology, Indiana University, Bloomington, Indiana 47405,USA. frederic.pontvianne@univ-perp.fr

Abstract

Eukaryotes can have thousands of 45S ribosomal RNA (rRNA) genes, many of which are silenced during development. Using fluorescence-activated sorting techniques, we show that active rRNA genes in Arabidopsis thaliana are present within sorted nucleoli, whereas silenced rRNA genes are excluded. DNA methyltransferase (met1), histone deacetylase (hda6), or chromatin assembly (caf1) mutants that disrupt silencing abrogate this nucleoplasmic-nucleolar partitioning. Bisulfite sequencing data indicate that active nucleolar rRNA genes are nearly completely demethylated at promoter CGs, whereas silenced genes are nearly fully methylated. Collectively, the data reveal that rRNA genes occupy distinct but changeable nuclear territories according to their epigenetic state.

KEYWORDS:

DNA methylation; RNA polymerase I; chromatin assembly; gene silencing; histone deacetylation; ribosomal RNA gene; transcription

PMID:
23873938
PMCID:
PMC3731543
DOI:
10.1101/gad.221648.113
[Indexed for MEDLINE]
Free PMC Article

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