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Rheumatology (Oxford). 2013 Oct;52(10):1769-74. doi: 10.1093/rheumatology/ket241. Epub 2013 Jul 19.

Association of Takayasu arteritis with HLA-B 67:01 and two amino acids in HLA-B protein.

Author information

1
Center for Genomic Medicine, Kyoto University Graduate School of Medicine, Kyoto 606-8507, Japan. a0001101@kuhp.kyoto-u.ac.jp.

Abstract

OBJECTIVE:

Takayasu arteritis (TAK) is a rare autoimmune arteritis that affects large arteries. Although the association between TAK and HLA-B 52:01 is established, the other susceptibility HLA-B alleles are not fully known. We performed genetic association studies to determine independent HLA-B susceptibility alleles other than HLA-B 52:01 and to identify important amino acids of HLA-B protein in TAK susceptibility.

METHODS:

One hundred patients with TAK and 1000 unrelated healthy controls were genotyped for HLA-B alleles in the first set, followed by a replication set containing 73 patients with TAK and 1000 controls to compare the frequencies of HLA-B alleles. Step-up logistic regression analysis was performed to identify susceptibility amino acids of HLA-B protein.

RESULTS:

Strong associations of susceptibility to TAK with HLA-B 52:01 and HLA-B 67:01 were observed (P = 1.0 × 10(-16) and 9.5 × 10(-6), respectively). An independent susceptibility effect of HLA-B 67:01 from HLA-B 52:01 was also detected (P = 1.8 × 10(-7)). Amino acid residues of histidine at position 171 and phenylalanine at position 67, both of which are located in antigen binding grooves of the HLA-B protein, were associated with TAK susceptibility (P ≤ 3.8 × 10(-5)) with a significant difference from other amino acid variations (ΔAIC ≥ 9.65).

CONCLUSION:

HLA-B 67:01 is associated with TAK independently from HLA-B 52:01. Two amino acids in HLA-B protein are strongly associated with TAK susceptibility.

KEYWORDS:

HLA-B; Takayasu arteritis; aortitis; genetic association study; vasculitis

PMID:
23873822
DOI:
10.1093/rheumatology/ket241
[Indexed for MEDLINE]

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