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Arch Gynecol Obstet. 2014 Feb;289(2):299-306. doi: 10.1007/s00404-013-2967-9. Epub 2013 Jul 20.

Non-invasively collected amniotic fluid as a source of possible biomarkers for premature rupture of membranes investigated by proteomic approach.

Author information

1
Department of Obstetrics and Gynecology, San Gerardo Hospital, MBBM Foundation, University of Milano-Bicocca, Via Pergolesi 33, 20900, Monza, MB, Italy, sara.consonni@tiscali.it.

Abstract

PURPOSE:

Preterm delivery is one of the main causes of perinatal morbidity and mortality and it accounts for 75 % of perinatal mortality and more than half of the long-term morbidity. We applied a proteomic approach based on mass spectrometry (MS) for biomarkers discovery of preterm premature rupture of membranes (pPROM) by investigating amniotic fluid (AF) invasively and non-invasively collected.

METHODS:

Amniotic fluid was obtained from vagina of women with pPROM (group 1), PROM at term (group 2) and by genetic amniocentesis (group 3). Pre-fractionated AF proteome was analyzed through matrix assisted laser desorption ionization-time of flight (MALDI-TOF) MS. The characterization of proteins/peptides of interest was obtained by high performance liquid chromatography-electrospray tandem MS.

RESULTS:

Three peptides overexpressed in pPROM and able to discriminate the groups 1 and 2 were detected. One peptide was identified as the fragment Gly452LAVPDGPLGLPPKPro466 of the protein KIAA1522, expressed by fetal brain and liver. This peptide was overexpressed in a patient of the group 3, completely asymptomatic at the time of the amniocentesis, who later developed pPROM.

CONCLUSION:

Amniotic fluid invasively and non-invasively collected can be analyzed by MALDI-TOF MS to obtain proteomic profiles. Proteomic analysis identified a peptide with promising diagnostic capability for pPROM.

PMID:
23872981
DOI:
10.1007/s00404-013-2967-9
[Indexed for MEDLINE]
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