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Anal Biochem. 2013 Oct 15;441(2):180-1. doi: 10.1016/j.ab.2013.06.023. Epub 2013 Jul 16.

Preparation of 2-nitro-5-thiobenzoate for the routine determination of reagent hypochlorous acid concentrations.

Author information

1
Winthrop University Hospital, Mineola, NY 11501, USA. Electronic address: Tjeitner@Winthrop.org.

Abstract

Solutions of hypochlorous acid (HOCl) decay over time. This decay indicates the necessity for methods and reagents for the routine measurement of this oxidant. 2-Nitro-5-thiobenzoate is commonly used to measure HOCl concentrations. This article describes a method for the preparation of 2-nitro-5-thiobenzoate that is stable for at least 3 months. This method relies on the partial rather than full reduction of 5,5'-dithiobis-(2-nitrobenzoic acid) and the resulting equilibrium between the substrate and the product.

KEYWORDS:

2-Nitro-5-thiobenzoate; Ellman’s reagent; Hypochlorous acid

PMID:
23872002
PMCID:
PMC4326040
DOI:
10.1016/j.ab.2013.06.023
[Indexed for MEDLINE]
Free PMC Article
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