Abstract
The protective effect of extract of Crataegus pinnatifida (Rosaceae) pollen (ECPP) on the DNA damage response to oxidative stress was investigated and assessed with an alkaline single-cell gel electrophoresis (SCGE) assay and pBR322 plasmid DNA breaks in site-specific and non-site-specific systems. Total phenolic content, total flavonoid content, individual phenolic compounds, antioxidant activities (1,1-diphenyl-2-picrylhydrazyl (DPPH), radical scavenging activity, FRAP, and chelating activity) were also determined. The results showed that ECPP possessed a strong ability to protect DNA from being damaged by hydroxyl radicals in both the site-specific system and the non-site-specific system. It also exhibited a cytoprotection effect in mouse lymphocytes against H₂O₂-induced DNA damage. These protective effects may be related to its high total phenolic content (17.65±0.97 mg GAE/g), total flavonoid content (8.04±0.97 mg rutin/g), strong free radical scavenging activity and considerable ferrous ion chelating ability (14.48±0.21 mg Na₂EDTA/g).
Keywords:
DNA damage; Extract of Crataegus pinnatifida pollen (ECPP); Oxidative stress; Phenolic compounds.
Copyright © 2013 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antioxidants / analysis
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Antioxidants / chemistry
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Antioxidants / isolation & purification
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Antioxidants / pharmacology*
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Biological Products / analysis
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Biological Products / chemistry
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Biological Products / isolation & purification
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Biological Products / pharmacology
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Comet Assay
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Crataegus / chemistry*
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DNA Breaks / drug effects
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DNA Damage / drug effects*
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Ethnopharmacology
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Flavonoids / analysis
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Flavonoids / isolation & purification
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Flavonoids / pharmacology
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Free Radical Scavengers / analysis
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Free Radical Scavengers / chemistry
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Free Radical Scavengers / isolation & purification
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Free Radical Scavengers / pharmacology
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Iron Chelating Agents / analysis
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Iron Chelating Agents / chemistry
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Iron Chelating Agents / isolation & purification
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Iron Chelating Agents / pharmacology
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Lymphocytes / drug effects*
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Lymphocytes / metabolism
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Medicine, Chinese Traditional
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Mice
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Mice, Inbred Strains
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Oxidative Stress / drug effects*
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Phenols / analysis
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Phenols / isolation & purification
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Phenols / pharmacology
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology*
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Pollen / chemistry*
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Propolis / chemistry
Substances
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Antioxidants
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Biological Products
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Flavonoids
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Free Radical Scavengers
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Iron Chelating Agents
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Phenols
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Plant Extracts
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Propolis