Protective effect of extract of Crataegus pinnatifida pollen on DNA damage response to oxidative stress

Food Chem Toxicol. 2013 Sep:59:709-14. doi: 10.1016/j.fct.2013.07.015. Epub 2013 Jul 16.

Abstract

The protective effect of extract of Crataegus pinnatifida (Rosaceae) pollen (ECPP) on the DNA damage response to oxidative stress was investigated and assessed with an alkaline single-cell gel electrophoresis (SCGE) assay and pBR322 plasmid DNA breaks in site-specific and non-site-specific systems. Total phenolic content, total flavonoid content, individual phenolic compounds, antioxidant activities (1,1-diphenyl-2-picrylhydrazyl (DPPH), radical scavenging activity, FRAP, and chelating activity) were also determined. The results showed that ECPP possessed a strong ability to protect DNA from being damaged by hydroxyl radicals in both the site-specific system and the non-site-specific system. It also exhibited a cytoprotection effect in mouse lymphocytes against H₂O₂-induced DNA damage. These protective effects may be related to its high total phenolic content (17.65±0.97 mg GAE/g), total flavonoid content (8.04±0.97 mg rutin/g), strong free radical scavenging activity and considerable ferrous ion chelating ability (14.48±0.21 mg Na₂EDTA/g).

Keywords: DNA damage; Extract of Crataegus pinnatifida pollen (ECPP); Oxidative stress; Phenolic compounds.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / analysis
  • Antioxidants / chemistry
  • Antioxidants / isolation & purification
  • Antioxidants / pharmacology*
  • Biological Products / analysis
  • Biological Products / chemistry
  • Biological Products / isolation & purification
  • Biological Products / pharmacology
  • Comet Assay
  • Crataegus / chemistry*
  • DNA Breaks / drug effects
  • DNA Damage / drug effects*
  • Ethnopharmacology
  • Flavonoids / analysis
  • Flavonoids / isolation & purification
  • Flavonoids / pharmacology
  • Free Radical Scavengers / analysis
  • Free Radical Scavengers / chemistry
  • Free Radical Scavengers / isolation & purification
  • Free Radical Scavengers / pharmacology
  • Iron Chelating Agents / analysis
  • Iron Chelating Agents / chemistry
  • Iron Chelating Agents / isolation & purification
  • Iron Chelating Agents / pharmacology
  • Lymphocytes / drug effects*
  • Lymphocytes / metabolism
  • Medicine, Chinese Traditional
  • Mice
  • Mice, Inbred Strains
  • Oxidative Stress / drug effects*
  • Phenols / analysis
  • Phenols / isolation & purification
  • Phenols / pharmacology
  • Plant Extracts / chemistry
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Pollen / chemistry*
  • Propolis / chemistry

Substances

  • Antioxidants
  • Biological Products
  • Flavonoids
  • Free Radical Scavengers
  • Iron Chelating Agents
  • Phenols
  • Plant Extracts
  • Propolis