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J Ethnopharmacol. 2013 Sep 16;149(2):506-12. doi: 10.1016/j.jep.2013.07.008. Epub 2013 Jul 18.

Inhibitory effect of Coptis chinensis on inflammation in LPS-induced endotoxemia.

Author information

1
Department of Prescriptionology, College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Rhizoma coptidis (RC) has been used as a remedy for inflammation-related diseases in traditional medicine. Although it is known to have anti-inflammatory activities, its mechanism of action on lipopolysaccharide (LPS)-induced inflammation has not yet been identified in detail.

AIM OF THE STUDY:

This study was designed to assess the beneficial effects of pretreatment with RC in ameliorating LPS-induced liver inflammation.

MATERIALS AND METHODS:

Mice were orally administered RC (500, 1000 mg/kg) for three days in a row. 1h after the last RC administration, the mice were intraperitoneally injected with LPS (35 mg/kg). After treatment, histological alterations and inflammatory factor levels in the liver and proinflammatory cytokines in the serum associated with inflammation were examined.

RESULTS:

We found that pretreatment with RC (500 and 1000 mg/kg) exerted a significant protective effect by attenuating liver histopathological changes in endotoxemic mice. The results also demonstrated that RC suppressed secretion of LPS-stimulated pro-inflammatory cytokines, such as interleukin-6 (IL-6). Furthermore, RC inhibited LPS-mediated nuclear factor (NF)-κB activation via the prevention of IκB-α phosphorylation, as well as the phosphorylation of ERK1/2, JNK, and p38 MAPKs. These results were associated with decreases in the expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (I-NOS).

CONCLUSIONS:

The results presented here clearly demonstrate that RC could significantly protect mice against LPS-induced acute liver injury.

KEYWORDS:

Coptis chinensis; IL-6; Lipopolysaccharide (LPS); MAPKs; NF-κB

PMID:
23871807
DOI:
10.1016/j.jep.2013.07.008
[Indexed for MEDLINE]

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